                               SEQUENCE LISTING

<110> UNIVERSITE CATHOLIQUE DE LOUVAIN
 
<120> ANTI-GARP PROTEIN AND USES THEREOF

<130> NLW/JCP1/P146120WO00

<140> PCT/IB2016/000182
<141> 2016-02-02

<150> 62/111,429
<151> 2015-02-03

<160> 66    

<170> PatentIn version 3.5

<210> 1
<211> 662
<212> PRT
<213> Homo sapiens

<400> 1
Met Arg Pro Gln Ile Leu Leu Leu Leu Ala Leu Leu Thr Leu Gly Leu 
1               5                   10                  15      


Ala Ala Gln His Gln Asp Lys Val Pro Cys Lys Met Val Asp Lys Lys 
            20                  25                  30          


Val Ser Cys Gln Val Leu Gly Leu Leu Gln Val Pro Ser Val Leu Pro 
        35                  40                  45              


Pro Asp Thr Glu Thr Leu Asp Leu Ser Gly Asn Gln Leu Arg Ser Ile 
    50                  55                  60                  


Leu Ala Ser Pro Leu Gly Phe Tyr Thr Ala Leu Arg His Leu Asp Leu 
65                  70                  75                  80  


Ser Thr Asn Glu Ile Ser Phe Leu Gln Pro Gly Ala Phe Gln Ala Leu 
                85                  90                  95      


Thr His Leu Glu His Leu Ser Leu Ala His Asn Arg Leu Ala Met Ala 
            100                 105                 110         


Thr Ala Leu Ser Ala Gly Gly Leu Gly Pro Leu Pro Arg Val Thr Ser 
        115                 120                 125             


Leu Asp Leu Ser Gly Asn Ser Leu Tyr Ser Gly Leu Leu Glu Arg Leu 
    130                 135                 140                 


Leu Gly Glu Ala Pro Ser Leu His Thr Leu Ser Leu Ala Glu Asn Ser 
145                 150                 155                 160 


Leu Thr Arg Leu Thr Arg His Thr Phe Arg Asp Met Pro Ala Leu Glu 
                165                 170                 175     


Gln Leu Asp Leu His Ser Asn Val Leu Met Asp Ile Glu Asp Gly Ala 
            180                 185                 190         


Phe Glu Gly Leu Pro Arg Leu Thr His Leu Asn Leu Ser Arg Asn Ser 
        195                 200                 205             


Leu Thr Cys Ile Ser Asp Phe Ser Leu Gln Gln Leu Arg Val Leu Asp 
    210                 215                 220                 


Leu Ser Cys Asn Ser Ile Glu Ala Phe Gln Thr Ala Ser Gln Pro Gln 
225                 230                 235                 240 


Ala Glu Phe Gln Leu Thr Trp Leu Asp Leu Arg Glu Asn Lys Leu Leu 
                245                 250                 255     


His Phe Pro Asp Leu Ala Ala Leu Pro Arg Leu Ile Tyr Leu Asn Leu 
            260                 265                 270         


Ser Asn Asn Leu Ile Arg Leu Pro Thr Gly Pro Pro Gln Asp Ser Lys 
        275                 280                 285             


Gly Ile His Ala Pro Ser Glu Gly Trp Ser Ala Leu Pro Leu Ser Ala 
    290                 295                 300                 


Pro Ser Gly Asn Ala Ser Gly Arg Pro Leu Ser Gln Leu Leu Asn Leu 
305                 310                 315                 320 


Asp Leu Ser Tyr Asn Glu Ile Glu Leu Ile Pro Asp Ser Phe Leu Glu 
                325                 330                 335     


His Leu Thr Ser Leu Cys Phe Leu Asn Leu Ser Arg Asn Cys Leu Arg 
            340                 345                 350         


Thr Phe Glu Ala Arg Arg Leu Gly Ser Leu Pro Cys Leu Met Leu Leu 
        355                 360                 365             


Asp Leu Ser His Asn Ala Leu Glu Thr Leu Glu Leu Gly Ala Arg Ala 
    370                 375                 380                 


Leu Gly Ser Leu Arg Thr Leu Leu Leu Gln Gly Asn Ala Leu Arg Asp 
385                 390                 395                 400 


Leu Pro Pro Tyr Thr Phe Ala Asn Leu Ala Ser Leu Gln Arg Leu Asn 
                405                 410                 415     


Leu Gln Gly Asn Arg Val Ser Pro Cys Gly Gly Pro Asp Glu Pro Gly 
            420                 425                 430         


Pro Ser Gly Cys Val Ala Phe Ser Gly Ile Thr Ser Leu Arg Ser Leu 
        435                 440                 445             


Ser Leu Val Asp Asn Glu Ile Glu Leu Leu Arg Ala Gly Ala Phe Leu 
    450                 455                 460                 


His Thr Pro Leu Thr Glu Leu Asp Leu Ser Ser Asn Pro Gly Leu Glu 
465                 470                 475                 480 


Val Ala Thr Gly Ala Leu Gly Gly Leu Glu Ala Ser Leu Glu Val Leu 
                485                 490                 495     


Ala Leu Gln Gly Asn Gly Leu Met Val Leu Gln Val Asp Leu Pro Cys 
            500                 505                 510         


Phe Ile Cys Leu Lys Arg Leu Asn Leu Ala Glu Asn Arg Leu Ser His 
        515                 520                 525             


Leu Pro Ala Trp Thr Gln Ala Val Ser Leu Glu Val Leu Asp Leu Arg 
    530                 535                 540                 


Asn Asn Ser Phe Ser Leu Leu Pro Gly Ser Ala Met Gly Gly Leu Glu 
545                 550                 555                 560 


Thr Ser Leu Arg Arg Leu Tyr Leu Gln Gly Asn Pro Leu Ser Cys Cys 
                565                 570                 575     


Gly Asn Gly Trp Leu Ala Ala Gln Leu His Gln Gly Arg Val Asp Val 
            580                 585                 590         


Asp Ala Thr Gln Asp Leu Ile Cys Arg Phe Ser Ser Gln Glu Glu Val 
        595                 600                 605             


Ser Leu Ser His Val Arg Pro Glu Asp Cys Glu Lys Gly Gly Leu Lys 
    610                 615                 620                 


Asn Ile Asn Leu Ile Ile Ile Leu Thr Phe Ile Leu Val Ser Ala Ile 
625                 630                 635                 640 


Leu Leu Thr Thr Leu Ala Ala Cys Cys Cys Val Arg Arg Gln Lys Phe 
                645                 650                 655     


Asn Gln Gln Tyr Lys Ala 
            660         


<210> 2
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VH-CDR1 peptide"

<400> 2
Gly Phe Ser Leu Thr Gly Tyr Gly Ile Asn 
1               5                   10  


<210> 3
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VH-CDR2 peptide"

<400> 3
Met Ile Trp Ser Asp Gly Ser Thr Asp Tyr Asn Ser Val Leu Thr Ser 
1               5                   10                  15      


<210> 4
<211> 13
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VH-CDR3 peptide"

<400> 4
Asp Arg Asn Tyr Tyr Asp Tyr Asp Gly Ala Met Asp Tyr 
1               5                   10              


<210> 5
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR1 peptide"

<400> 5
Lys Ala Ser Asp His Ile Lys Asn Trp Leu Ala 
1               5                   10      


<210> 6
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR2 peptide"

<400> 6
Gly Ala Thr Ser Leu Glu Ala 
1               5           


<210> 7
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR3 peptide"

<400> 7
Gln Gln Tyr Trp Ser Thr Pro Trp Thr 
1               5                   


<210> 8
<211> 140
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      heavy chain variable region polypeptide"

<400> 8
Met Ala Val Leu Ala Leu Leu Phe Cys Leu Val Thr Phe Pro Ser Cys 
1               5                   10                  15      


Ile Leu Ser Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala 
            20                  25                  30          


Pro Ser Gln Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu 
        35                  40                  45              


Thr Gly Tyr Gly Ile Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Leu Gly Met Ile Trp Ser Asp Gly Ser Thr Asp Tyr Asn Ser 
65                  70                  75                  80  


Val Leu Thr Ser Arg Leu Arg Ile Ser Lys Asp Asn Ser Asn Ser Gln 
                85                  90                  95      


Val Phe Leu Lys Met Asn Ser Leu Gln Val Asp Asp Thr Ala Arg Tyr 
            100                 105                 110         


Tyr Cys Ala Arg Asp Arg Asn Tyr Tyr Asp Tyr Asp Gly Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 
    130                 135                 140 


<210> 9
<211> 127
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      light chain variable region polypeptide"

<400> 9
Met Lys Phe Pro Ser Gln Leu Leu Leu Phe Leu Leu Phe Arg Ile Thr 
1               5                   10                  15      


Gly Ile Ile Cys Asp Ile Gln Val Thr Gln Ser Ser Ser Tyr Leu Ser 
            20                  25                  30          


Val Ser Leu Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Asp His 
        35                  40                  45              


Ile Lys Asn Trp Leu Ala Trp Tyr Gln Gln Lys Pro Gly Ile Ala Pro 
    50                  55                  60                  


Arg Leu Leu Val Ser Gly Ala Thr Ser Leu Glu Ala Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Lys Asn Phe Thr Leu Ser Ile Thr 
                85                  90                  95      


Ser Leu Gln Thr Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Tyr Trp 
            100                 105                 110         


Ser Thr Pro Trp Thr Phe Gly Gly Gly Thr Thr Leu Glu Ile Arg 
        115                 120                 125         


<210> 10
<211> 420
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      heavy chain variable region polynucleotide"

<400> 10
atggctgtcc tggcattact cttctgcctg gtaacattcc caagctgtat cctttcccag       60

gtgcagctga aggagtcagg acctggcctg gtggcgccct cacagagcct gtccatcaca      120

tgcaccgtct cagggttctc attaaccggc tatggtataa actgggttcg ccagcctcca      180

ggaaagggtc tggagtggct gggaatgata tggagtgatg gaagcacaga ctataattca      240

gttctcacat ccagactgag gatcagtaag gataattcca atagccaggt tttcttaaaa      300

atgaacagtc tgcaagttga tgacacagcc aggtactatt gtgccagaga tcgaaactac      360

tatgattacg acggggctat ggactactgg ggtcaaggaa cctcagtcac cgtctcctca      420


<210> 11
<211> 381
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      light chain variable region polynucleotide"

<400> 11
atgaagtttc cttctcaact tctgctcttc ctgctgttca gaatcacagg cataatatgt       60

gacatccagg tgacacaatc ttcatcctac ttgtctgtat ctctaggaga cagggtcacc      120

attacttgca aggcaagtga ccacattaaa aattggttag cctggtatca gcagaaacca      180

ggaattgctc ctaggctctt agtttctggt gcaaccagtt tggaagctgg ggttccttca      240

agattcagtg gcagtggatc tggaaagaat ttcactctca gcattaccag tcttcagact      300

gaagatgttg ctacttatta ctgtcaacag tattggagta caccgtggac gttcggtgga      360

ggcaccactc tggagatcag a                                                381


<210> 12
<211> 41
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      conformational epitope polypeptide"

<400> 12
His Leu Ser Leu Ala His Asn Arg Leu Ala Met Ala Thr Ala Leu Ser 
1               5                   10                  15      


Ala Gly Gly Leu Gly Pro Leu Pro Arg Val Thr Ser Leu Asp Leu Ser 
            20                  25                  30          


Gly Asn Ser Leu Tyr Ser Gly Leu Leu 
        35                  40      


<210> 13
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VH-CDR1 LHG-10 peptide"

<400> 13
Ser Tyr Tyr Ile Asp 
1               5   


<210> 14
<211> 17
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VH-CDR2 LHG-10 peptide"

<400> 14
Arg Ile Asp Pro Glu Asp Gly Gly Thr Lys Tyr Ala Gln Lys Phe Gln 
1               5                   10                  15      


Gly 
    


<210> 15
<211> 17
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VH-CDR3 LHG-10 peptide"

<400> 15
Asn Glu Trp Glu Thr Val Val Val Gly Asp Leu Met Tyr Glu Tyr Glu 
1               5                   10                  15      


Tyr 
    


<210> 16
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR1 LHG-10 peptide"


<220> 
<221> VARIANT
<222> (5)..(5) 
<223> /replace="Thr"

<220> 
<221> VARIANT
<222> (7)..(7) 
<223> /replace="Val"

<220> 
<221> VARIANT
<222> (9)..(9) 
<223> /replace="Phe"

<220>
<221> misc_feature
<222> (1)..(11)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<400> 16
Gln Ala Ser Gln Ser Ile Ser Ser Tyr Leu Ala 
1               5                   10      


<210> 17
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR2 LHG-10 peptide"


<220> 
<221> VARIANT
<222> (1)..(1) 
<223> /replace="Arg"

<220> 
<221> VARIANT
<222> (2)..(2) 
<223> /replace="Thr"

<220> 
<221> VARIANT
<222> (4)..(4) 
<223> /replace="Ile"

<220> 
<221> VARIANT
<222> (5)..(5) 
<223> /replace="Pro"

<220> 
<221> VARIANT
<222> (6)..(6) 
<223> /replace="Lys"

<220>
<221> misc_feature
<222> (1)..(7)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<400> 17
Gly Ala Ser Arg Leu Gln Thr 
1               5           


<210> 18
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR3 LHG-10 peptide"


<220> 
<221> VARIANT
<222> (4)..(4) 
<223> /replace="Ala" or "Tyr" or "Val"

<220> 
<221> VARIANT
<222> (6)..(6) 
<223> /replace="Leu" or "Val"

<220> 
<221> VARIANT
<222> (8)..(8) 
<223> /replace="Pro"

<220>
<221> misc_feature
<222> (1)..(9)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<400> 18
Gln Gln Tyr Asp Ser Ala Pro Val Thr 
1               5                   


<210> 19
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR1 LHG-10 peptide"

<400> 19
Gln Ala Ser Gln Ser Ile Ser Ser Tyr Leu Ala 
1               5                   10      


<210> 20
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR2 LHG10 peptide"

<400> 20
Gly Ala Ser Arg Leu Gln Thr 
1               5           


<210> 21
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR3 LHG10 peptide"

<400> 21
Gln Gln Tyr Asp Ser Leu Pro Val Thr 
1               5                   


<210> 22
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR1 LHG10.3 peptide"

<400> 22
Gln Ala Ser Gln Ser Ile Val Ser Tyr Leu Ala 
1               5                   10      


<210> 23
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR2 LHG10.3 peptide"

<400> 23
Gly Ala Ser Arg Leu Gln Thr 
1               5           


<210> 24
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR3 LHG10.3 peptide"

<400> 24
Gln Gln Tyr Ala Ser Ala Pro Val Thr 
1               5                   


<210> 25
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR1 LHG10.4 peptide"

<400> 25
Gln Ala Ser Gln Ser Ile Ser Ser Tyr Leu Ala 
1               5                   10      


<210> 26
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR2 LHG10.4 peptide"

<400> 26
Gly Thr Ser Arg Leu Lys Thr 
1               5           


<210> 27
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR3 LHG10.4 peptide"

<400> 27
Gln Gln Tyr Tyr Ser Ala Pro Val Thr 
1               5                   


<210> 28
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR1 LHG10.5 peptide"

<400> 28
Gln Ala Ser Gln Thr Ile Ser Ser Phe Leu Ala 
1               5                   10      


<210> 29
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR2 LHG10.5 peptide"

<400> 29
Arg Ala Ser Ile Pro Gln Thr 
1               5           


<210> 30
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR3 LHG10.5 peptide"

<400> 30
Gln Gln Tyr Val Ser Ala Pro Pro Thr 
1               5                   


<210> 31
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR1 LHG10.6 peptide"

<400> 31
Gln Ala Ser Gln Ser Ile Ser Ser Tyr Leu Ala 
1               5                   10      


<210> 32
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR2 LHG10.6 peptide"

<400> 32
Gly Ala Ser Arg Leu Lys Thr 
1               5           


<210> 33
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VL-CDR3 LHG10.6 peptide"

<400> 33
Gln Gln Tyr Ala Ser Val Pro Val Thr 
1               5                   


<210> 34
<211> 126
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      heavy chain variable region of sequence of LHG10 polypeptide"

<400> 34
Glu Val Gln Leu Val Gln Pro Gly Ala Glu Leu Arg Asn Ser Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Arg Phe Thr Ser Tyr 
            20                  25                  30          


Tyr Ile Asp Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Arg Ile Asp Pro Glu Asp Gly Gly Thr Lys Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Phe Thr Ala Asp Thr Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Val Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Glu Trp Glu Thr Val Val Val Gly Asp Leu Met Tyr Glu 
            100                 105                 110         


Tyr Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210> 35
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      Light chain variable region of sequence LHG10 polypeptide"

<400> 35
Asp Ile Gln Met Thr Gln Ser Pro Thr Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Arg Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Ser Phe Thr Leu Thr Ile Ser Gly Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Gly Thr Tyr Tyr Cys Gln Gln Tyr Asp Ser Leu Pro Val 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys 
            100                 105         


<210> 36
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      Light chain variable region of sequence of LHG10.3 
      polypeptide"

<400> 36
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Val Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Arg Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Ser Phe Thr Leu Thr Ile Ser Gly Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Gly Thr Tyr Tyr Cys Gln Gln Tyr Ala Ser Ala Pro Val 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Gly Val Glu Leu Lys 
            100                 105         


<210> 37
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      Light chain variable region of sequence of LHG10.4
      polypeptide"

<400> 37
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Gly Thr Ser Arg Leu Lys Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Ser Phe Thr Leu Thr Ile Ser Gly Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Gly Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ser Ala Pro Val 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys 
            100                 105         


<210> 38
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      Light chain variable region of sequence of LHG10.5 
      polypeptide"

<400> 38
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Pro Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Thr Ile Ser Ser Phe 
            20                  25                  30          


Leu Ala Trp Tyr His Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Arg Ala Ser Ile Pro Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Ser Phe Thr Leu Thr Ile Gly Gly Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Gly Thr Tyr Tyr Cys Gln Gln Tyr Val Ser Ala Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys 
            100                 105         


<210> 39
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      Light chain variable region of sequence of LHG10.6
      polypeptide"

<400> 39
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ala Ser Gln Ser Ile Ser Ser Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Asn Ile Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Arg Leu Lys Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Ser Phe Thr Leu Thr Ile Ser Gly Leu Glu Ala 
65                  70                  75                  80  


Glu Asp Ala Gly Thr Tyr Tyr Cys Gln Gln Tyr Ala Ser Val Pro Val 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys 
            100                 105         


<210> 40
<211> 45
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      human GARP truncated and tagged polypeptide"

<400> 40
Glu Ala Ala Glu Asn Leu Tyr Phe Gln Gly Ala Ala Trp Ser His Pro 
1               5                   10                  15      


Gln Phe Glu Lys Gly Ala Ala Trp Ser His Pro Gln Phe Glu Lys Gly 
            20                  25                  30          


Ala Ala Trp Ser His Pro Gln Phe Glu Lys Gly Ala Ala 
        35                  40                  45  


<210> 41
<211> 4216
<212> DNA
<213> Macaca fascicularis

<220>
<221> source
<223> /note="predicted cynomolgus GARP"

<400> 41
ggtggggcag ctgagtggcc tgcgcctcct cgggccgtga ccccggggtc tggcgcgggg       60

tgggacccgg gggcgggttt gcgcaaaatg tgccgagact gcccgggaga ggaactgcgg      120

ccgcgctgag ccagagccat gagcccccag atcctgctgc tcctggccct gctgacccta      180

ggcctggctg cacaacacca agacaaagtg gcatgtaaga tggtggacaa gaaggtctcg      240

tgccagggtc tgggcctgct ccaggtcccc ttggtgctcc cgccggacac tgagaccctt      300

gatctctctg ggaaccagct gcggagtatc ctggcctcac ccctgggctt ctacacggca      360

cttcgtcacc tggacctgag caccaatgag atcaacttcc tccagccagg agccttccag      420

gccctgaccc acctggagca cctcagcctg gctcacaacc ggctggcgat ggccactgcg      480

ctgagtgccg gtggtctggg ccccctgcca cgtgtgacct ccctggacct gtctgggaac      540

agcctgtaca gcggcctgct ggagcggctg ctaggggagg cacccagcct gcataccctc      600

tcactggcgg agaacagtct gactcgcctc acccgccaca ccttccggga catgcctgcg      660

ctggagcagc ttgacctgca tagcaacgtg ctgatggaca tcgaggatgg cgccttcgag      720

ggcctgcccc acctgaccca tctcaacctt tccaggaatt ccctcacctg catctccgac      780

ttcagccttc agcagctgcg ggtgctggac ctgagctgca acagcattga ggcctttcag      840

acggcctccc agccccaggc cgagttccag ctcacctggc ttgacctgcg ggagaacaaa      900

ctgctccatt tccccgacct ggccgcgctc ccgagactca tctacctgaa cttgtccaac      960

aacctcatcc ggctccccac agggccaccc caggacagca agggcatcca cgcgccttcc     1020

gagggctggt cagccctgcc cctctcaacc cccaatggga atgtcagtgc ccgccccctt     1080

tcccagctct tgaatctgga tttgagctac aatgagattg aactcatccc cgacagcttt     1140

cttgagcacc tgacctccct gtgcttcctg aacctcagca gaaactgctt gcggaccttt     1200

gaggcccggc gctcaggctc cctgccctgc ctgatgctcc ttgatttaag ccacaatgcc     1260

ctggagacac tggaactggg cgccagagcc ctggggtcct tgcggacact gctcctacag     1320

ggcaatgccc tgcgggacct gcctccatac acctttgcca acctggccag cctgcagcgg     1380

ctcaacctgc aggggaaccg ggtcagcccc tgtggggggc cgaatgagcc cggccccgcc     1440

agctgtgtgg ccttctctgg catcgcctcc ctccgcagcc tgagcctggt ggataatgag     1500

atagagctgc tcagggcagg ggccttcctc cataccccac tgactgagct ggacctttct     1560

tccaaccctg ggctggaggt ggccacaggg gccttgacag gcctggaggc ctccttggaa     1620

gtcctggcac tgcagggcaa tgggttgacg gtcctgcagg tggacctgcc ctgcttcatc     1680

tgcctcaagc ggctcaatct tgccgagaac cgcctgagcc accttcccgc ctggacacag     1740

gctgtgtcac tggaggtgct ggacctgcga aacaacagct tcagcctcct gccaggcagt     1800

gccatgggtg gcctggagac cagcctccgg cgcctctacc tgcaggggaa tccactcagc     1860

tgctgtggca atggctggct ggcagcccag ctgcaccagg gccgtgtgga cgtggacgcc     1920

acccaggacc tgatctgccg cttcagctcc caggaggagg tgtccctgag ccacgtgcgt     1980

cccgaggact gtgagaaggg ggggctcaag aacatcaacc tcatcatcat cctcaccttc     2040

atactggtct ctgccatcct cctcaccacg ctggccacct gctgctgtgt ccgccggcag     2100

aagtttaacc aacagtataa agcctaaaga agccgggaga cactctaggt caatggggga     2160

gcctgaggta cagagaagag tgaggactga ctcaaggtca cacagtgacc caggatccca     2220

gaactctggt ctccaaattg caacccggga cacctttctc tgccgcctgc tgcatcagcg     2280

ggtgaccccc ttcccgggct gcactttggg tccagctgtg gaagccagaa gttgggcggt     2340

ttcagggaca gcagggaata atgttgacct atcagatcaa caaatcttca ctgagcatct     2400

actttgtgcc acactctgct ctgggcactg ggaatgctgg gaaataagat acactcccgc     2460

cctcaagaat ctcccagtct ggtaggaggg agtgctacag agccgcgtgg tgaccacgca     2520

gtgtgcttag ggcctgaggt gtgaaagccc gggactccgg agctcggcag gccccgctgg     2580

ttcgatgcga agagtcctgc cccagccatg ccagggtgag agagggccaa gcctgggagg     2640

atttgtctga gacatttcca agcagactgt ttgtcacatc ttctgataat gactttcagt     2700

ctctctgaaa atgaaaagct tatgaccgga agagagaatt ggagccatac gagtgtgtct     2760

tggatctggt gctgttaggc gggccgcggc ggctccagca gggtctggtt aaggggtcca     2820

gcccggcact ggaccattcc gtctcctgct ctggacaggc cgtctccctg cctggcactc     2880

tcatgttaca cagcctgatc ccagtactgc tctaagcgcc gtccctgccc agcccttctc     2940

catcgcagcc ccgccttggc tgctaagcca agagctaaaa ccttagatat ctgattctgt     3000

tttgcaccca gcttggcaga tgtggatgtg aatccaagcc tgtgtctgcc cctatatgac     3060

agcccttgga ggttggtatt ttatccccat tttataaaag aggaaactga agttctacaa     3120

atctccttcc agggccccag ctaactaatg ccttaggtga gattcaaacc ctcatacttc     3180

tgtctccagg gcctgatctt tgccactgca ggggctgcag gccgttaagt ggacaggaag     3240

tggctccaca tagcccgagc agggtctgga agtatcctgt gctatgcata cctgctctct     3300

cctctctccc aggcaggcag ctgcaggcgc tctcctcctc ctctgcctta gtttccctcc     3360

ttccatcctt tccaccctgg tgtgggttct cctgttctct ctgtgctctt gcatgctctc     3420

attccctttt cctctattga gcagagcctg gagtttgaga ctattgaatc caacctcccc     3480

attgcacaga tggggaaact gaggcttagg aagagaatga aacttgtgga gagctataca     3540

gagcctctgg ggaaaaaaaa gagagcccta tttggggatg agattagggg ttggaccata     3600

gtgatgtcct ctcttggctg tcacatcaca agataatgct ggctccaaac ttcctttctg     3660

tgcctcctca tgcaggattt tttttttccc tcttggaaaa ataggtagaa aggctcaccc     3720

agataacccc ctatccctca tagcatggag tcatgagctg tctgggaaga atggacacgc     3780

tgggaccaac tcaagacctt gtgttgctgt cttcatcatc ttacctgtgc ttggcccaca     3840

gtctggctca tgatgtgggc tcagtaatgt gcaagaaaat gaaaatgcca ctctctccac     3900

cccattttac agaggagaac accgaggccc agaggaagtt aagggagagt caatgggcag     3960

agccagggct aggccctggt gatgtgtgga gcacccaggc agacccagtc ctggttggga     4020

tcacaaccac aggtgctact gcacgtgaca ctcttcctta ggcctggagg ccaaggtgtg     4080

ggtcctcacg cctgatcttt gaaaacacta cacaatgctg ctgtcagttc ccaggaccca     4140

ggccgcagcc caggcctcgg gaccaactct ttgtataacc tacctgaatg tattaaaaac     4200

taattttgga gaagca                                                     4216


<210> 42
<211> 2188
<212> DNA
<213> Macaca fascicularis

<220>
<221> source
<223> /note="cynomolgus TGF beta-1"

<400> 42
ccgccgccct tcgcgccctg ggccatctcc ctcccacctc cctccgcgga tcagccagac       60

tgcgagggcc ccggccgggg gcagggggga cgccccgtcc ggggcacccc cccggctctg      120

agccgcccgc ggggccggcc tcggcccgga gcggaggaag gagtcgccga ggagcagcct      180

gaggccccag agtctgagac gagccgccgc cgcccccgcc actgcgggga ggagggggag      240

gaggagcggg aggagggacg agctggtcgg gagaagagga aaaaaacttt tgagactttt      300

ccgttgccgc tgggagccgg aggcgcgggg acctcttggc gcgacgctgc cccgcgagga      360

ggcaggactt ggggacccca gaccgcctcc ctttgccgcc ggggacgctt gctccctccc      420

tgccccctac acggcgtccc tcaggcgccc ccattccgga ccagccctcg ggagtcgccg      480

acccggcctc ccgcaaatac ttttccccag acctcgggcg caccccctgc acgccgcctt      540

catccccggc ctgtctcctg agcccccgcg catcctagac cttttctcct ccaggagacg      600

gatctctctc cgacctgcca cagatcccct attcaagacc acccaccttc tggtaccaga      660

tctcgcccat ctaggttatt tccgtgggat actgagacac ccccggtcca agcctcccct      720

ccaccactgc gcccttctcc ctgaggacct caactttccc tcgaggccct cctacctttt      780

cccgggagac ccccagcccc tgcaggggcg gggcctcccc accacgctag ccctgttcgc      840

cctctcggca gtgccggggg gcgccgcctc ccccatgccg ccctccgggc tgcggctgct      900

gccgctgctg ctaccgctgc tgtggctact ggtgctgacg cctggccggc cggccgccgg      960

actatccacc tgcaagacta tcgacatgga gctggtgaag cggaagcgca tcgaggccat     1020

ccgcggccag atcctgtcca agctgcggct cgccagcccc ccgagccagg gggaggtgcc     1080

gcccggcccg ctgcccgagg ccgtgctcgc cctgtacaac agcacccgcg accgggtggc     1140

cggggagagt gcggagccgg aacccgaacc ggaggccgac tactacgcca aggaggtcac     1200

ccgcgtgcta atggtggaaa cccacaacga aatctatgac aagttcaagc agagcacaca     1260

cagcatatat atgttcttca acacatcaga gctccgagaa gcagtacctg aacctgtgtt     1320

gctctcccgg gcagagctgc gtctgctgag gctcaagtta aaagtggagc agcatgtgga     1380

gctgtaccag aaatacagca acaattcctg gcgatacctc agcaaccggc tgctggcgcc     1440

cagcgactcg ccggagtggt tgtcttttga tgtcaccgga gttgtgcggc agtggttgag     1500

ccgcggaggg gaaattgagg gctttcgcct tagcgcccac tgctcctgtg acagcaaaga     1560

taacacactg caagtggaca tcaacgcagg gttcactacc ggccgccgag gtgacctggc     1620

caccattcat ggcatgaacc ggcctttcct gcttctcatg gccaccccgc tggagagggc     1680

ccaacatctg caaagctccc ggcaccgccg agccctggac accaactact gcttcagctc     1740

cacggagaag aactgctgcg tgcggcagct gtatattgac ttccgcaagg acctcggctg     1800

gaagtggatc cacgagccca agggctacca tgccaacttc tgcctgggac cctgccccta     1860

catttggagc ctggacacgc agtacagcaa ggtcctggcc ctgtacaacc agcataaccc     1920

gggcgcctcg gcggcgccgt gctgcgtgcc gcaggcgctg gagccgctgc ccatcgtgta     1980

ctacgtgggc cgcaagccca aggtggagca gctgtccaac atgatcgtgc gctcctgcaa     2040

atgcagctga ggccccgtcc cgccccgccc caccccggca ggcccggccc cgccccgccc     2100

cgcccccgct gccttgccct tgggggctgt atttaaggac acccgtgccc caagcccacc     2160

tggggcccca ttaaagatgg agagagga                                        2188


<210> 43
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      cyno TGFB S1 primer"

<400> 43
cgcctccccc atgccgccct ccg                                               23


<210> 44
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      cyno TGFB Sense 2 primer"

<400> 44
acaattcctg gcgatacctc                                                   20


<210> 45
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      cyno TGFB Anti-Sense 1 primer"

<400> 45
ctcaaccact gccgcacaac                                                   20


<210> 46
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      cyno TGFB Anti-Sense 2 primer"

<400> 46
tcagctgcat ttgcaggagc                                                   20


<210> 47
<211> 231
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      human IgG1; Fc region of LHG-10 polypeptide"

<400> 47
Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro 
1               5                   10                  15      


Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210> 48
<211> 330
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      Heavy chain constant domain of LHG-10 polypeptide"


<220> 
<221> VARIANT
<222> (180)..(180) 
<223> /replace="Gln"

<220>
<221> misc_feature
<222> (1)..(330)
<223> /note="Variant residues given in the sequence have no 
      preference with respect to those in the annotations
      for variant positions"

<400> 48
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210> 49
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      Light chain constant domain of LHG-10 polypeptide"

<400> 49
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210> 50
<211> 121
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      heavy chain variable region polypeptide"

<400> 50
Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Gly Tyr 
            20                  25                  30          


Gly Ile Asn Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Met Ile Trp Ser Asp Gly Ser Thr Asp Tyr Asn Ser Val Leu Thr 
    50                  55                  60                  


Ser Arg Leu Arg Ile Ser Lys Asp Asn Ser Asn Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Asn Ser Leu Gln Val Asp Asp Thr Ala Arg Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Arg Asn Tyr Tyr Asp Tyr Asp Gly Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120     


<210> 51
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      light chain variable region polypeptide"

<400> 51
Asp Ile Gln Val Thr Gln Ser Ser Ser Tyr Leu Ser Val Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Asp His Ile Lys Asn Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Ile Ala Pro Arg Leu Leu Val 
        35                  40                  45              


Ser Gly Ala Thr Ser Leu Glu Ala Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Lys Asn Phe Thr Leu Ser Ile Thr Ser Leu Gln Thr 
65                  70                  75                  80  


Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Tyr Trp Ser Thr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Thr Leu Glu Ile Arg 
            100                 105         


<210> 52
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      VH-CDR1 peptide"

<400> 52
Gly Tyr Gly Ile Asn 
1               5   


<210> 53
<211> 390
<212> PRT
<213> Homo sapiens

<220>
<221> source
<223> /note="TGF-beta1 amino acid sequence"

<400> 53
Met Pro Pro Ser Gly Leu Arg Leu Leu Pro Leu Leu Leu Pro Leu Leu 
1               5                   10                  15      


Trp Leu Leu Val Leu Thr Pro Gly Arg Pro Ala Ala Gly Leu Ser Thr 
            20                  25                  30          


Cys Lys Thr Ile Asp Met Glu Leu Val Lys Arg Lys Arg Ile Glu Ala 
        35                  40                  45              


Ile Arg Gly Gln Ile Leu Ser Lys Leu Arg Leu Ala Ser Pro Pro Ser 
    50                  55                  60                  


Gln Gly Glu Val Pro Pro Gly Pro Leu Pro Glu Ala Val Leu Ala Leu 
65                  70                  75                  80  


Tyr Asn Ser Thr Arg Asp Arg Val Ala Gly Glu Ser Ala Glu Pro Glu 
                85                  90                  95      


Pro Glu Pro Glu Ala Asp Tyr Tyr Ala Lys Glu Val Thr Arg Val Leu 
            100                 105                 110         


Met Val Glu Thr His Asn Glu Ile Tyr Asp Lys Phe Lys Gln Ser Thr 
        115                 120                 125             


His Ser Ile Tyr Met Phe Phe Asn Thr Ser Glu Leu Arg Glu Ala Val 
    130                 135                 140                 


Pro Glu Pro Val Leu Leu Ser Arg Ala Glu Leu Arg Leu Leu Arg Leu 
145                 150                 155                 160 


Lys Leu Lys Val Glu Gln His Val Glu Leu Tyr Gln Lys Tyr Ser Asn 
                165                 170                 175     


Asn Ser Trp Arg Tyr Leu Ser Asn Arg Leu Leu Ala Pro Ser Asp Ser 
            180                 185                 190         


Pro Glu Trp Leu Ser Phe Asp Val Thr Gly Val Val Arg Gln Trp Leu 
        195                 200                 205             


Ser Arg Gly Gly Glu Ile Glu Gly Phe Arg Leu Ser Ala His Cys Ser 
    210                 215                 220                 


Cys Asp Ser Arg Asp Asn Thr Leu Gln Val Asp Ile Asn Gly Phe Thr 
225                 230                 235                 240 


Thr Gly Arg Arg Gly Asp Leu Ala Thr Ile His Gly Met Asn Arg Pro 
                245                 250                 255     


Phe Leu Leu Leu Met Ala Thr Pro Leu Glu Arg Ala Gln His Leu Gln 
            260                 265                 270         


Ser Ser Arg His Arg Arg Ala Leu Asp Thr Asn Tyr Cys Phe Ser Ser 
        275                 280                 285             


Thr Glu Lys Asn Cys Cys Val Arg Gln Leu Tyr Ile Asp Phe Arg Lys 
    290                 295                 300                 


Asp Leu Gly Trp Lys Trp Ile His Glu Pro Lys Gly Tyr His Ala Asn 
305                 310                 315                 320 


Phe Cys Leu Gly Pro Cys Pro Tyr Ile Trp Ser Leu Asp Thr Gln Tyr 
                325                 330                 335     


Ser Lys Val Leu Ala Leu Tyr Asn Gln His Asn Pro Gly Ala Ser Ala 
            340                 345                 350         


Ala Pro Cys Cys Val Pro Gln Ala Leu Glu Pro Leu Pro Ile Val Tyr 
        355                 360                 365             


Tyr Val Gly Arg Lys Pro Lys Val Glu Gln Leu Ser Asn Met Ile Val 
    370                 375                 380                 


Arg Ser Cys Lys Cys Ser 
385                 390 


<210> 54
<211> 249
<212> PRT
<213> Homo sapiens

<220>
<221> source
<223> /note="LAP amino acid sequence"

<400> 54
Leu Ser Thr Cys Lys Thr Ile Asp Met Glu Leu Val Lys Arg Lys Arg 
1               5                   10                  15      


Ile Glu Ala Ile Arg Gly Gln Ile Leu Ser Lys Leu Arg Leu Ala Ser 
            20                  25                  30          


Pro Pro Ser Gln Gly Glu Val Pro Pro Gly Pro Leu Pro Glu Ala Val 
        35                  40                  45              


Leu Ala Leu Tyr Asn Ser Thr Arg Asp Arg Val Ala Gly Glu Ser Ala 
    50                  55                  60                  


Glu Pro Glu Pro Glu Pro Glu Ala Asp Tyr Tyr Ala Lys Glu Val Thr 
65                  70                  75                  80  


Arg Val Leu Met Val Glu Thr His Asn Glu Ile Tyr Asp Lys Phe Lys 
                85                  90                  95      


Gln Ser Thr His Ser Ile Tyr Met Phe Phe Asn Thr Ser Glu Leu Arg 
            100                 105                 110         


Glu Ala Val Pro Glu Pro Val Leu Leu Ser Arg Ala Glu Leu Arg Leu 
        115                 120                 125             


Leu Arg Leu Lys Leu Lys Val Glu Gln His Val Glu Leu Tyr Gln Lys 
    130                 135                 140                 


Tyr Ser Asn Asn Ser Trp Arg Tyr Leu Ser Asn Arg Leu Leu Ala Pro 
145                 150                 155                 160 


Ser Asp Ser Pro Glu Trp Leu Ser Phe Asp Val Thr Gly Val Val Arg 
                165                 170                 175     


Gln Trp Leu Ser Arg Gly Gly Glu Ile Glu Gly Phe Arg Leu Ser Ala 
            180                 185                 190         


His Cys Ser Cys Asp Ser Arg Asp Asn Thr Leu Gln Val Asp Ile Asn 
        195                 200                 205             


Gly Phe Thr Thr Gly Arg Arg Gly Asp Leu Ala Thr Ile His Gly Met 
    210                 215                 220                 


Asn Arg Pro Phe Leu Leu Leu Met Ala Thr Pro Leu Glu Arg Ala Gln 
225                 230                 235                 240 


His Leu Gln Ser Ser Arg His Arg Arg 
                245                 


<210> 55
<211> 112
<212> PRT
<213> Homo sapiens

<220>
<221> source
<223> /note="mature TGF-beta1 amino acid sequence"

<400> 55
Ala Leu Asp Thr Asn Tyr Cys Phe Ser Ser Thr Glu Lys Asn Cys Cys 
1               5                   10                  15      


Val Arg Gln Leu Tyr Ile Asp Phe Arg Lys Asp Leu Gly Trp Lys Trp 
            20                  25                  30          


Ile His Glu Pro Lys Gly Tyr His Ala Asn Phe Cys Leu Gly Pro Cys 
        35                  40                  45              


Pro Tyr Ile Trp Ser Leu Asp Thr Gln Tyr Ser Lys Val Leu Ala Leu 
    50                  55                  60                  


Tyr Asn Gln His Asn Pro Gly Ala Ser Ala Ala Pro Cys Cys Val Pro 
65                  70                  75                  80  


Gln Ala Leu Glu Pro Leu Pro Ile Val Tyr Tyr Val Gly Arg Lys Pro 
                85                  90                  95      


Lys Val Glu Gln Leu Ser Asn Met Ile Val Arg Ser Cys Lys Cys Ser 
            100                 105                 110         


<210> 56
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      FOXP3i1 sense primer"

<400> 56
aaacctacta caaaacaaaa caac                                              24


<210> 57
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      FOXP3i1 antisense primer"

<400> 57
ggaggaagag aagagggta                                                    19


<210> 58
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      FOXP3i1 probe primer"

<400> 58
cctataaaat aaaatatcta ccctc                                             25


<210> 59
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      demethylated FOXP3i1 sense primer"

<400> 59
tctaccctct tctcttcctc ca                                                22


<210> 60
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      demethylated FOXP3i1 antisense primer"

<400> 60
gatttttttg ttattgatgt tatggt                                            26


<210> 61
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      demethylated FOXP3i1 probe primer"

<400> 61
aaacccaaca catccaacca                                                   20


<210> 62
<211> 41
<212> PRT
<213> Homo sapiens

<400> 62
His Leu Ser Leu Ala His Asn Arg Leu Ala Met Ala Thr Ala Leu Ser 
1               5                   10                  15      


Ala Gly Gly Leu Gly Pro Leu Pro Arg Val Thr Ser Leu Asp Leu Ser 
            20                  25                  30          


Gly Asn Ser Leu Tyr Ser Gly Leu Leu 
        35                  40      


<210> 63
<211> 41
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 63
His Leu Ser Leu Ala His Asn Arg Leu Ala Thr Gly Met Ala Leu Ser 
1               5                   10                  15      


Ala Gly Gly Leu Gly Pro Leu Pro Arg Val Thr Ser Leu Asp Leu Ser 
            20                  25                  30          


Gly Asn Ser Leu Tyr Ser Gly Leu Leu 
        35                  40      


<210> 64
<211> 41
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 64
His Leu Ser Leu Ala His Asn Arg Leu Ala Met Ala Thr Ala Leu Ser 
1               5                   10                  15      


Ala Gly Gly Leu Gly Pro Leu Pro Leu Leu Val Ser Leu Asp Leu Ser 
            20                  25                  30          


Gly Asn Ser Leu Tyr Ser Gly Leu Leu 
        35                  40      


<210> 65
<211> 41
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 65
His Leu Ser Leu Ala His Asn Arg Leu Ala Met Ala Thr Ala Leu Ser 
1               5                   10                  15      


Ala Gly Gly Leu Gly Pro Leu Pro Arg Val Thr Ser Leu Asp Leu Ser 
            20                  25                  30          


Gly Asn Ser Leu His Gly Asn Leu Leu 
        35                  40      


<210> 66
<211> 41
<212> PRT
<213> Mus sp.

<400> 66
His Leu Asn Leu Ala His Asn Arg Leu Ala Thr Gly Met Ala Leu Asn 
1               5                   10                  15      


Ser Gly Gly Leu Gly Arg Leu Pro Leu Leu Val Ser Leu Asp Leu Ser 
            20                  25                  30          


Gly Asn Ser Leu His Gly Asn Leu Val 
        35                  40      


